Total oxidant/antioxidant status, paraoxonase, aryesterase activities and PON1 192Q/R phenotyping

The relation between oxidative stress and pathogenesis of atherosclerosis has been shown with numerous studies. Antioxidant properties of paraoxonase 1 enzyme has been center of interest in recent years. In this study we investigated total antioxidant status, oxidant status, oxidative stres index and their relation with paraoxonase, arylesterase and PON1 192 Q/R phenotyping in patients with coronary artery disease.Patients who had coronary artery disease and no coronary artery disease that have been diagnosed with anjiography and healthy controls were recruited into the study. Blood samples were taken and total oxidant status, total antioxidant status, oxidative stress index, paraoxonase activity, arylesterase activity, PON1 192Q/R phenotyping, lipid hydroperoxide levels and lipid profile were analysed.Serum total oxidant status and antioxidant status were significantly higher in both patient groups when compared with the healthy group (p<0.05). There was no significant difference in terms of oxidative stress index between groups (p>0.05). Although arylesterase activity was significantly lower in both patient groups when compared with the healthy controls (p<0.05), there was no significant difference in paraoxonase activity between three groups (p>0.05). At the same time there was no statistically significant corelation between coronary artery disease and PON1 192Q/R phenotyping (p>0.05).In conclusion, our findings have suggested that oxidative status increases in patients with coronary artery disease and antioxidant status increases as a compansatuary mechanism against this situation. Also this study has demonstrated that arylesterase activity is decreased related to HDL and arylyesterase is more affected than paraoxonase activity in etiopatogenesis of coronary artery disease. In addition, this study has shown that there is no relation between coronary artery disease and PON1 Q/R phenotyping and RR phenotype is more susceptible to oxidative stress.Key words: Arylesterase, Coronary artery disease, Oxidative stress, PON1 192Q/R phenotyping, Total antioxidant status, Total oxidant status.