Kronik sosyal izolasyon modelinde agmatinin etkilerinin araştırılması

Aim of study: Agmatine is an endogenous molecule that is widely available in the central nervous system and has neurotransmitter/neuromodulator functions. Previous studies researched the antidepressant and anxiolytic effects of agmatine so this study aimed to investigate its effects on chronic social isolation model (SI). Material and methods: Sprague Dawley rats were separated to 4 groups (n=8/group) as control, SI, SI+Fluoxetine (FLU) and SI+Agmatine (AGM). Animals were isolated in individual cages for 5 weeks. Right after that, control and and SI groups were given serum physiologic, while SI+FLU and SI+AGM groups were given 15mg / kg FLU or 40mg / kg AGM for 10 days as one daily intraperitoneal dose. Open field (OF), elevated plus maze (EPM), novel objects recognition (NOR) and marble burying (MB) tests were applied. All animals were decapitated and prefrontal cortex dissection was performed for molecular analysis. Results: SI group when compared to the control group showed less total activity in OF test, more entrances and time spent into closed arms in EPM test, corruption in the discrimination index in NOR test and exessive burying in MB test. These changes were partially suppressed in FLU therapy group while totally suppressed in AGM therapy group. The elevated expression of IL-1B and NF-kB mRNA shown in SI group was also suppressed partially in FLU therapy group and totally in AGM therapy group. Conclusion: This study shows that agmatine expresses effects against changes caused by SI and these effects may be partially caused by the suppression of proinflammatory cytokines production. Keywords: Agmatine, fluoxetine, social isolation, proinflammatory cytokines