Evaluation of drug release properties of curcumin loaded mesoporous organosilica nanocarrier and its therapeutic effect in cancer treatment

Within the scope of the thesis, a new drug delivery system was synthesized by using the oxamide bridged bissilyl compound that can undergo enzymatic degradation. Biodegradability, drug loading, drug release and cytotoxic properties of the synthesized drug carrier system on the HT29 colon cell line were studied. Curcumin, a natural product, was used to determine drug loading and release properties. While the structural determinations of the synthesized organic substances were carried out by 1H, 13C NMR, FT-IR, and mass analysis, the structural properties of the drug delivery system DDS were determined by FT-IR, XRD, SEM, EDS, TEM, TGA and N2 sorption analyzes. UV-Vis spectroscopy was used to determine drug loading and release properties. The oxamide bridged bissilyl compound was obtained by the reaction of oxalyl chloride and 2-((3-(triethoxysilyl)propyl)disulfanyl)aniline compound (1). The drug delivery system DDS was obtained as a result of the co-condensation reaction of oxamide bridged bissilyl compound (2) and 1,4-bis(triethoxysilyl)benzene. The reference nanocarrier PMO was also synthesized. As a medicine, the natural product curcumin has been loaded into DDS and PMO materials. The loading rate was determined as 18% for DDS and 0,13 for PMO. Curcumin-loaded drug delivery system Cur@DDS was determined to leave less than 1% of the cargo carried without pore-capping agents at physiological and endosomal pH. The drug release properties of Cur@DDS were investigated in the presence and absence of trypsin protein at physiological pH. The results showed that the percentage of drug release in the presence of trypsin was higher than the percentage of drug release in the medium without trypsin. This result showed that the enzyme sensitive mesoporous organosilica nanocarrier DDS was successfully synthesized. The cytotoxic effect of Cur@DDS and Cur@PMO on the HT29 colon cell line was determined to be 0,10 on average.