Mapping AFLP and SNP markers in lentile genome by using recombinant inbred lines

One hundred one individuals from RIL mapping population derived from a cross between Precoz X WA8649041 were genotyped by using SNP and AFLP markers. This study aimed to develop high-density genetic linkage map of lentil RIL population composed of SNP and AFLP markers. SNP markers were determined by using Illumina sequencing platform-GBS protocol in RIL population and its parents. As a result of sequencing, 6688 SNP out of 7758 SNP markers were scored and in this thesis study first 400 SNPs were used to make linkage map. In this study, a total of 194 polymorphic band were obtained from 6 AFLP primer combinations and used in mapping. Precoz X WA8649041 population genome was mapped by using Joinmap 4.0 software and covered 413 cM. In this study, 9 different linkage group of which sizes were ranging from 73.8 cM to 16.9 cM were obtained. The mean length of linkage groups were determined as 45.9 and 37 markers were obtained per linkage group. An average marker density of 1 marker per 0.9 cM were provided. Among the scored markers, 321 SNP (77%) and 162 AFLP (9%) markers were able to be mapped on linkage groups. The maximum number of markers in one linkage group was 77 (LG3), the minimum was just 10 (LG9). This map made by using SNP and AFLP markers in RIL populations and its parents can be a reference linkage map for lentil mapping studies which has large genome size (~4 Gbp). The linkage map obtained as a result of this study can be used for marker assisted selection (MAS) analysis in lentil breeding studies.