Detection of methamphetamine and MDMA (ECSTASY) in urine and hair samples by chromatographic methods

Nowadays, use of amphetamine type stimulants (methamphetamine, MDMA etc.) and economic, social and health problems are getter increasing among young people and adults due to asy to reach and low cost. Therefore, right, fast and reliable toxicological analysis in biological samples of these abused drugs have gained great importance. In this study, the methods were developed for the determination of methamphetamine and MDMA in urine by GC-MS and in hair by LC-MS/MS. Liquid-liquid extraction method (Toxi Tube A ) was used in toxicological analysis of these substances in urine by GC-MS and the method was optimized. Optimum values were obtained with 2 ml urine volume, hydrolysis temperature of 60 °C and 30 min. of hydrolysis time, 70 °C of derivatization temperature and 30 min. of derivatization time. The optimized method was validated for selectivity, recovery (methamphetamine= 87,7-% to 113.1%, MDMA= 88,2-% to 99,7%), intra-day and inter-day repeatability (100 µg/ L, 5 days, n=3, methamphetamine= % RSD 5,8 MDMA= % RSD 3,1%), linearity (methamphetamine= 25 to 1000 µg /L linear, MDMA= 50-2000 µg/L linear), LOD (methamphetamine=5,6 µg /L, MDMA=8,33 µg/L), LOQ (methamphetamine=18,5 µg /L, MDMA=27,8 μg / L), stability (4 hours stable) and the matrix effect parameters. In this study, method was developed for the determination of methamphetamine and MDMA in 10 mg hair using solid-phase extraction method by LC-MS MS. The method was validated for selectivity, recovery (methamphetamine=71,8%, MDMA=75,0%), linearity (methamphetamine and MDMA 25 to 2000 pg /mg linear), LOD (methamphetamine=1,3 pg/mg, MDMA=40,9 pg/mg), LOQ (methamphetamine=4,0 pg/mg, MDMA=123,8 pg/mg), and the matrix effect parameters. The methods developed by the study has been successfully applied to urine and hair samples taken from several drug users.