Norduz koyununda büyüme hormonu (GH) geni polimorfizmleri

Genetic markers and their applications to animal molacular genetics have commonly been used to determine polymorphisms. Restriction Fragment Length Polymorphism (RFLP) is a molecular method of genetic analysis exploiting variations in homologous DNA sequences, consequently being considered as one of the informative genetic markers.The growth hormone (GH) gene plays major roles in growth regulation and development of higher organisms and the growth traits are complex traits involving multiple genes, loci and interactions. The aim of the research was to detect GH polymorphisms in Norduz sheep. Genomic DNAs were isolated from blood samples of Norduz sheep and GH1 primers based on the 5 'flanking sequence of the sheep GH1 gene (Gene Bank Accession Number: D00476) designed by (Hua et al., 2009) and GH gene designed by (Kuulasma, 2002) were amplified. The amplified PCR product with the length of 422 bp for GH1 locus and 934 bp for GH locus. Both loci were digested with HaeIII restriction enzyme and the results displayed the presence of GH1/HaeIII homozygous AA genotype with fragments 366 bp and 56 bp and heterozyogous AB genotype with fragments 422 bp and 366 bp. However, homozygous BB genotype did not appear. Considering GH/HaeIII digestion, all expected genotypes AA, AB, BB were detected. Additionally, the population under investigation did not exhibite to be in Hardy-Weinberg equilibrium (HWE) for both loci. The present study concluded that GH1and GH/HaeIII could be used as a genetic marker in sheep for growth characters.