Characterization and purification of polyphenol oxidase enzyme from marabel and marfona potato varieties which are grown in Ödemiş (İzmir) district

In this study, polyphenol oxidase (PPO) enzymes from Marabel and Marfona potato varieties grown in Ödemiş (İzmir) district were purified and characterized. PPO enzymes were purified from Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity gel from two potato varieties and the degree of purification and percentage yields were determined. The purity of the enzymes was checked by SDS-PAGE electrophoresis gel. Using the catechol substrate, the optimum pH value of PPO enzyme in Marabel was 7 and the optimum temperature value was 20 °C, and the optimum pH value of PPO enzyme in Marfona potatoes was 7 and the optimum temperature value was 25 °C. KM and Vmax values of purified polyphenol oxidase enzymes were determined using Linewear-Burk graph equation. KM and Vmax values of PPO enzymes in purified Marabel and Marfona potato varieties were determined at optimum conditions as 5.4 mM, 769.23 U/mLmin and 6.7 mM, 3333.33 U/mLmin, respectively.