The effect of omega-3 and omega-6 fatty acids on apoptosis related genes in human breast cancer MCF7 cell line
2019
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Advisor: Doç. Dr. Makbule Baylan ; Doç. Dr. Mehmet Bertan Yılmaz
Abstract (EN)
In this study, various doses and combinations of omega-3 (EPA, DHA) and omega-6 (LA) fatty acids in human breast cancer, MCF7 cell line were administered for 24 hours. As a result of these applications, viability of the cells was controlled by applying MTT method in MCF7 cell line. In the subsequent stages, protein isolation was performed from the treated cells. Enzyme-linked immunosorbent assay (ELISA) was used to determine the expression levels of apoptosis-related genes, phospho-p53 (Ser15), p53, Bad, phospho-Bad (Ser112), cleaved Caspase-3 (Asp175), and cleaved PARP (Asp214) protein. Not all doses treated resulted in significant differences in phospho-p53 (Ser15) and p53 expressions, but significantly increased expression of other proteins.
Author
Fatma Ezgi Öztecik
Institution
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Fatma Ezgi Öztecik (Master Thesis). The effect of omega-3 and omega-6 fatty acids on apoptosis related genes in human breast cancer MCF7 cell line, 2019, Çukurova University.
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