The effects of Omeprazole on ileum, stomach fundus, colon and bladder functional responses in Lipopolysaccharide induced sepsis model in rats

Sepsis causes an uncontrolled systemic inflammatory response to infection. Proton pump inhibitor omeprazole (OME) is known to have anti-inflammatory effects beyond the suppression of acid secretion in cells and organs. In order to investigate the effect of changes in smooth muscle functional responses in LPS-induced rat sepsis model and OME treatment, acetylcholine (ACh), electrical field stimulation (EFS) and nonadrenergic noncholinergic (NANC) contraction responses in gastric fundus, ileum, distal colon and bladder detrusor preparations were examined in organ bath. ACh and EFS contraction responses were obtained by incubating OME in the organ bath and the direct effect on tissue was examined. The effect of OME on survival in sepsis was evaluated. Omeprazole incubation decreased ACh responses in gastrointestinal tract (GIT) tissues and increased in bladder detrusor muscle. In the LPS-induced sepsis model, ACh responses in GIT tissues, EFS and EFS-induced NANC contraction responses in colon and bladder were increased. OME treatment had a positive effect on survival in the LPS-induced sepsis model and reversed increased ACh contraction responses in the colon. OME injection reduced the NANC contraction responses of EFS and EFS in the ileum and bladder tissues. Ethanol used as the solvent, reduced ACh contraction responses in gastric fundus, colon and bladder tissues independent of sepsis. These results showed that functional responses of smooth muscles were impaired in LPS-induced rat sepsis model. It has shown that OME treatment, with its positive effects on survival, reverses these disturbances, especially in the distal colon, and causes changes in functional responses in other tissues. In conclusion, OME may play a more important role in the clinical practice in the treatment of sepsis.