Detection of anti-CA II antibodies in autoimmune diseases

8. SUMMARYDetection of Anti-CA II Antibodies in Autoimmune DiseasesAutoimmune diseases arise from the immune responses of the body to its ownantigens. In 1991, the presence of anti-CA II antibody has been first reported byInagaki and et al. in the serums of patients with systemic lupus erythematosus andSjögren?s syndrome. After this year, anti-CA II antibodies were shown to exist inseveral autoimmune diseases by ELISA and Western blotting techniques.Here, it has been intended to establish an ELISA method in our laboratories inorder to be able to determine the anti-CA II antibodies for some autoimmune diseases.For this purpose, by using the serums of the patients with endometriosis in which thepresence of anti-CA II antibody had been formerly detected by Western blotting,performance evaluation for the ELISA method has been made. In addition, anti-CA IIantibody levels have been detected in the serums of 8 patients with endometriosis and20 patients with. The presence of anti-CA II antibody. In the serums of 3 of 8 patientswith endometriosis (37.5%) and 10 of 20 patients with rheumatoid arthritis (50%) havebeen shown the presence of anti-CA II antibody.In conclusion, it has been seen that the presence of anti-CA II antibodies forautoimmune diseases could be detected by this ELISA techniqueKeywords: Anti-CA II antibody, ELISA, rheumatoid arthritis, endometriosis