The activities of trpm2 ion channel and chrm1 in ovarian hyperstimulation syndrome (OHSS)

THE ACTIVITIES OF TRPM2 ION CHANNEL AND CHRM1 IN OVARIAN HYPERSTIMULATION SYNDROME (OHSS) In this study, TRPM2 ion channels and CHRM1 activities were investigated in the pathophysiology of ovarian hyperstimulation syndrome (OHSS). 22 days old 14 immature female Wistar albino rats divided into 2 groups randomly. 25 of them received 10 IU FSH for four days subcutenously and 30 IU human chorionic gonodotropin at fifth day for augmenting ovarian hyperstimulation. Group 1 (n = 7) normal for 27 days old, Group 2 (n = 7) rats were divided into two groups as 27 days old OHSS. Ovaries and blood samples were taken from all groups and rats were sacrificed. TRPM2 and CHRM1 activity in tissues and VEGF, IL1, IL6, IL10, TNFα , MDA levels in blood were measured. IL1 and IL6 levels were similar in two groups and no statistically significant difference was observed (p>0,05). When compared with the Control group, IL10 (p=0,038), TNFα (p=0,007), VEGF (p=0,0017) and MDA (p=0,004) levels were increased in the OHSS group. When compared with the Control group, CHRM1 (p=0,001) and TRPM2(p=0,001) levels were increased in the OHSS group's ovarian tissue. As a result, many different mediators and systems are responsible for pathology of OHSS. Increases in TRPM2 ion channels and CHRM1 activity provide a different perspective on the pathophysiology of OHSS.