Ultrastructural changes on spermatozoa considering the exposure period after pentoxifylline i̇mplementation

Ultrastructural Changes on Spermatozoa Considering the Exposure Period After Pentoxifylline İmplementation Various pharmacological agents have been defined to increase spermatozoon motility on the men whom have spermatozoon motility problem. Pentoxifylline is the most used agent in assisted reproduction technics. The most cummon usage area of pentoxifylline on male infertility is the stimulation of in vitro spermatozoon motility. The live spematozoon selection is important on intra cytoplasmic spermatozoa injection (ICSI) and in vitro fertilization which are assisted reproduction techniques, this selection is directly proportionate to spermatozoon motility. In assisted reproduction technics, with the realization of the importance of motility on in vitro fertilization capacity, the pentoxifylline usage in routine increased and it caused to be made of many studies on this issue. In our study, we the ultrastructural of different exposure-term pentoxifylline-treated normospermi samples in transmission electron microscopy (TEM) level. The normospermi samples that we specified, were incubated with pentoxifylline at different durations. The specified samples were analyzed by comparing the ultrastructural of spermatozoon at TEM level after the glycocalyx in the spermatozoona's membrane structure became clear by implementing the red rutenyum colouring. Then the samples were compared with control group. As a result, it's seen that while there is not too much distortion on morphological structure of spermatozoon samples that exposed to pentoxifylline for 5 minutes, there occurred significant morphological distortions on the spermatozoon samples that exposed to pentoxifylline for 60 minutes. Accordingly, the effect of pentoxifylline's period on spermatozoon morphology has been shown.