Phyllanthus emblica yüklü yara örtüsü malzemeleri

Since ancient times, plants have played a vital role in therapeutic approaches for a variety of diseases and ailments. Phyllanthus emblica (P. emblica) has been described in many studies to have multiple therapeutic activities and is considered an important part of Ayurvedic and Unani medicinal systems. This study sought to examine the efficacy of synthesized Phyllanthus emblica loaded cryogels on wound healing and their potential in wound dressing applications. For this purpose, polyvinylalcohol/gelatin (PVA/Gel) based cryogels were synthesized containing 0.5% (PVA/Gel/P.emblica-0.5), 1% (PVA/Gel/P.emblica-1), 1.5% (PVA/Gel/P.emblica-1.5), 2% (PVA/Gel/P.emblica-2) P. emblica extract. Swelling tests, Brunauer-Emmett-Teller (BET) and morphology analysis were conducted within the scope of characterization studies. In-vitro cell culture studies were performed using HaCaT cells to illustrate the suitability of the synthesized cryogels as wound dressing material. The swelling tests and BET results revealed that P. emblica loaded PVA/Gel cryogels was increased with increasing the amounts of P. emblica. Morphological results displayed that the cryogels had an dense, interconnected pore morphology and a macroporous structure. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue exclusion and live-dead assay results revealed that adding of P. emblica into cryogels led to enhanced cell proliferation, increased cell number, and improved cell viability. Based on the phase contrast microscope, scanning electron microscope (SEM), immunofluorescence and giemsa images of the HaCaT cells on P. emblica loaded PVA/Gel cryogels, it was observed that P. emblica promoted cell attachment, proliferation and penetration. In conclusion, it has been shown that PVA/Gel/P.emblica cryogels are suitable for use as wound dressing materials and can be developed with further studies.