Evaluation of picea orientalis resin extract as a cavity disinfectant: In vitro study

In this study, it is aimed to make chemical analysis of Picea orientalis resin extract, to determine its antimicrobial activity and to determine its bond strenght to dentin. Picea orientalis resins were dissolved with ethanol and their chemical analysis was done by HPLC-DAD method. Afterwards, antimicrobial activity was determined on selected oral bacteria by agar diffusion test method. 180 dentin samples prepared for bond strength test were randomly divided into 3 groups, one of which was the control group, according to the different cavity disinfectants [Chlorhexidine gluconate (Ceraxidin-C, Imicryl) and Picea orientalis resin extract] to be used. The dentin samples assigned to each group were divided into 2 more subgroups, using the same universal adhesive system (Single Bond Universal, 3M ESPE) in accordance with different surface preparation methods (self etch/etch&rinse). All groups were restored with the same composite system (Estelite Sigma Quick, Tokuyama Dental) using a clear plastic mold with a diameter of 3 mm and a height of 4 mm. Then half of the groups; It was subjected to aging process to examine the short and long term bond strength. Caffeic acid, ferulic acid and t-cinnamic acid contents of resin extracts were found to be high. The extracts showed high antimicrobial activity on S. aureus. Bond strength of unaged samples was statistically significantly higher than aged samples in all experimental groups (p:0.000; p>0.05). In the unaged samples, the bond strength of the control group was found to be significantly higher than that of the chlorhexidine (p:0.000) and Picea orientalis resin extract (p:0.000) groups, and the highest binding value was obtained (p<0.05). There was no significant difference between Chlorhexidine and Picea orientalis resin extract in all experimental groups (p>0.05). Resin extracts have rich phenolic and high antioxidant capacity and the highest antimicrobial activity is found on S. aureus. The use of resin extracts as a disinfectant does not adversely affect the binding values. The aging process decreases the binding values.