Microbiological investigation of the contents of saliva and evaluation in terms of decay incidence in polycystic ovary syndrome patients

The aim of this study is to examine the effects of Polycystic Ovary Syndrome (PCOS), which is common in women of reproductive age, on saliva, teeth and gingival tissues in these women. One-hundred individuals who were / were not diagnosed with PCOS in Gaziantep University Medical Faculty Hospital, Department of Obstetrics and Gynecology, and who were / were not diagnosed with insulin resistance in Gaziantep University Medical Faculty Endocrinology Department were included in this study (n=100). Subsequently, individuals, with PCOS and insulin resistance (PCOSID +), with PCOS and non-insulin resistance (PCOSID-), without PCOS and insulin resistance (ControlID +),and without PCOS and non-insulin resistance (ControlID-) were divided into 4 groups by retrospectively analyzing the results of their biochemical parameters (n=25). Sociodemographic data of the groups were evaluated with the anamnesis. DMFT (Decayed, Missing, Filled Teeth) index and periodontal indexes were used for dental and gingival health evaluation, while pH meter was used for saliva pH measurement. Also, Streptococcus Mutans (S. Mutans) numbers were analyzed by the real-time Polymerase Chain Reaction (PCR) method. In statistical analysis; One-way analysis of variance (ANOVA) and LSD tests were used for normally distributed variables while Kruskal-Wallis and pairwise tests were used for non-normally distributed variables. In the comparison of categorical variables between groups, Pearson Chi-square and Fisher's exact test test were used. Pearson correlation test was used to calculate the correlation of numerical variables normally distribution, while Spearman rho test was used for numerical variables non-normally distribution. p<0.05 was considered significant. In the comparison among the groups, a significant differences were found in terms of decayed, missing, filled teeth (DMFT) index, Plaque and Gingival indexes (PI and GI respectively), Streptococcus. Mutans (S. Mutans), salivary pH and sociodemographic data values (p<0.05). Among the compared groups, the highest DMFT index, S. Mutans, PI and GI values were found in the PCOSID (+) group, the lowest in the ControlID (-) group, while the lowest saliva pH value was found in the PCOSID (+) group. PCOSID (+) group was observed a significant correlation between S. Mutans and DMFT index values (p<0.05). There was a significant correlation between the unstimulated saliva flow rate and S. Mutans in all groups (p <0.05). Within the results, high S. Mutans and DMFT values were found in the saliva of patients with PCOS, which is a multifactorial syndrome, and it was determined that sociodemographic, salivary, and periodontal parameters affect thiscondition. Insulin resistance, which plays a central role in the pathogenesis of PCOS and oral microflora, was found to affect the parameters we investigated.