Investigation of the mitochondrial biogenesis pathways in premature ovarian failure rat models

Premature ovarian failure (POF) is defined as the presence of inadequate ovarian function before the age of 40 years. Reactive oxygen species (ROS) and mitochondrial biogenesis play an important role in this process. The aim of the project was to determine the role of AP39 in the prevention of adverse effects of cisplatin, stress and the role of mitochondrial biogenesis pathways in the development of POY. Sixty female Sprague Dawley rats were divided into control, AP39, cisplatin, cisplatin + AP39, stress and stress + AP39 groups that with 10 animals in each group. POF was formed with 1,5 mg / kg cisplatin or immobilization stress. Moris water maze was performed to detect the spatial learning and memory. Hormone levels, height, weight, overweight, estrus cycling follow, ROS, mitochondrial biogenesis gene expression levels were determined by different laboratuary methods. It were detected the significant decreases in height, weight, overweight and serum E2 levels and the significant increase in serum FSH and LH values in cisplatin and stress groups according to control. Mitochondrial biogenesis, over-related genes, spatial learning and memory were found to be significantly altered in both cisplatin and stress groups compared to controls, but AP39 was not normalizing all parameters in cisplatin+AP39 and stress+AP39 groups than cisplatin and stress groups.