Determination of total mitogenom sequences and gene location in Psorodonotus venosus ve Anterastes babadaghi ( Orthoptera, Tettigoniida )

DNA sequences of the mitochondrial genome frequently have been used as one of the main data sources in population genetics, phylogeny, phylogeography and other similar evolutionary studies. Orthoptera is one of the most species rich order of Insecta and mitochondrial genome of several species have been studies especially during last 2-3 years. This thesis includes data on mitochondrial genome of Psorodonotus venosus ve Anterastes babadaghi belonging to Tettigoniinae (Orhoptera, Tettigoniidae) obtained via next generation sequencing and evaluated by bioinformatics approaches. The total mitogenome of Psorodonotus venosus was 15842 base pairs and that of Anterastes babadaghi was 15883 base pairs. As in the most of the other eukaryotes the mitochondrial genome of these two species consists of 22 tRNA genes, 2 rRNA genes, 13 protein coding genes and the A+T rich control region. The arrangement of 37 genes in the mitochondrial genome of these two species is as in gene location in Pancrustacea which also considered as ancestral gene arrangement of Insecta. The AT/GC content in protein coding genes was % 68/32 and % 70/30 in Psorodonotus venosus and Anterastes babadaghi respectively. Data of this study indicates following results: (i) mitogenome of both species exhibit characteristics of multicelular organisms in several aspects, (ii) The gene location of mitochondrial genome of these two species representing Tettigoniinae is as in other hexapods, (iii) and this indicate that no gene arrangement has occurred in evolutionary history of Tettigoniinae, (iv) Both species have AT rich genome as in other orthopterans, and (v) the tRNA genes in Tettigoniinae exhibit some subfamily specific aspects such as the length and anti-codon of the genes and their overlapping or having non-coding bases with the adjacent genes, and (vi) similarly the length, the initial and stop codons of the genes and their overlapping or having non-coding bases with the adjacent genes in protein coding also exhibit subfamily specific patterns.