Investigation of anticancerogenic effects of radiolabeled indole-3-carbinol and PLGA encapsulated indole-3carbinol compounds utilizing in vitro methods

Recently, the usage of natural products has gained more importance and recent studies emphasized that consuming cruciferous vegetables (broccoli, brussel sprout, cauliflower, collard greens and curly kale) lowers cancer risk. In the literature there are a lot of studies which are showed that indol-3-karbinol (I3C) and metabolic ingredients of cruciferous vegetables provide antitumor activity against many cancer types including breast cancer, prostate cancer and colon cancer. In present study, radioiodination and encapsulation with poly lactic-co-glycolic acid (PLGA) of I3C has been performed. Anticancerogenic effects of I3C and PLGAencapsulated I3C (I3C-PLGA) have been investigated utilizingin vitromethods. Nanoparticles containing biodegradable and biocompatible polymers such as PLGA have been widely used and important research subject about pharmaceutical carriers. Their main usages in medical science contain being a carrier for drug,genes and antigens as well as in vitro studies. In this study, quality control of I3C and I3C-PLGA has been performed with High Performance Liquid Chromatography (HPLC) and structural analysis of 127I-I3C has been verified using Nuclear Magnetic Resonance (NMR). I3C and I3C-PLGA compounds have been radiolabeled with 131I[(131I-I3C), (131I-I3C-PLGA)] which is widely used for diagnosis and treatment in nuclear medicine. Radiolabelling yields ofradioiodinated compounds have been determined using Thin Layer Radio-Chromatography (TLRC) while anticancerogenic effects have been studied on breast adenocarcinoma epithelial(MCF-7), colon adenocarcinoma epithelial (Caco-2), prostate carcinoma epithelial (PC3) cells. All experimental results demonstrated that radiolabelled compounds are promising in order to be used in nuclear medicine as well as present study contributed previously reported studies.