The effect of benfotiamine and n-acetylcysteine in autologous intraperiyoneal transplantation of the ovary in rats

One of the most important unresolved issues in the ovary transplantation is prevention of ischemia reperfusion injury. Therefore, application of antioxidants to reduce free radical generation after ovarian transplant can be regarded as an important drug target against the consequences of ischemia and reperfusion injury. In this study, using N-acetylcysteine and benfotiamine over tranplantation effect of N-acetylcysteine and benfotiamine were investigated against injury induced by transplantation. N-acetylcysteine, is a N-acetylated species of natural amino acid L-cysteine. Acetylcysteine is a mucolytic agent and endogenous antioxidants, free radical scavengers the structure of sisteinproglutaty. As a glutathione precursor feeds glutathione pool during oxidative stress, glutathione redox cycle, provides a good defense system in the endothelium protection. Benfotiamine is a fat-soluble form of Vitamin B1. Benfotiamine as inhibitor on reactive oxygen species it has been shown in some studies. In the study, total number of 28 Wistar Albino 12-14 week old female rats used and were divided into 4 groups. Group I was used as the control group. Group II was used as the transplant group. Group III 1 day before and after the transplantation for 3 weeks was administered by oral gavage 75 mg / kg / day benfotiamine. Grup IV 1 day before and after the transplantation for 7 days 150 mg/kg/day N-acetylcysteine was administered intraperitoneally. The all group rats were decapitated under anesthesia at the end of the experimental period. After decapitation ovarian tissues of the rats removed quickly, then detected by appropriate fixative passed through the histologic follow-up series and embedded in parafin wax. The tissue sections from paraffin blocks Masson trichrome stain by applying analyzed histopathological differences between the groups. In addion to apoptotic cells were detected by applying the TUNEL technique in this section. Also with the aim look at the total antioxidant status (TAS) -Total oxidant status (TOS) activities in tissues, tissues were stored at -80 ° C until the working day. When compared with the control group in the transplant group was observed apoptotic cells in ovarian tissue, fibrosis, oxidative damage, a significant increase in levels of inflammatory cells. When benfotiamine and NAC-treated group compared with transplantation group, observed by a significant decrease at these changings. In terms of ovarian reserve in all groups compared with the control group, the number of primordial follicles was reduced but no significant difference between treatment groups. There was no difference between groups in terms secondary and primary follicles, corpus luteum. As a result; the ovarian transplantation caused increased apoptosis, necrosis, fibrosis as a result of oxidative damage caused by ischemia-reperfusion. Benfotiamine and NAC applications were found equally and effectively reduce this damage. Keywords: N-acetylcysteine, Benfothiamin, Ovarian transplantation