Investigation of antiepileptic effects of cloxyquin in pentylenetetrazole experimental rat model of epilepsy

In the presented study it is considered that, activation of TRESK (TWIK related spinally-expressed potassium channels), which is member of the the twopore potassium channel family, with its specific activator cloxyquin may display on antiepileptic effects by stabilizing neuronal membrane potential and making difficult to stimulate. The possible antiepileptic effects of cloxyquine were investigated by using the acute experimental epilepsy model which induced with pentylentetrazole. Seventy-two male Wistar rats which were randomly divided into nine groups used in study. Saline, dimethyl sulfoxide, valproic acid and five different doses of cloxyquine were administered to the groups. After acute epilepsy was generated and video recordings taken; the first myoclonic jerk, latency and seizure durations, the number of seizures, behavioral scores, survival rates of animals were examined. Chi-square, Shapiro-Wilk, Kruskal Wallis, Mann Whitney U tests, ROC, simple linear regression and The Spearman rank-order correlation coefficient analyzes were used for statistical analysis. In the study, there were no statistically differences between the groups in terms of seizure stages, first myoclonic jerk and latency durations. The most significant results in regard to better seizure stages, low number of seizures and not existing any death cases were seen in the groups which valproic acid (100 mg/kg) and 10 mg/kg of cloxyquin were administered. Survival rates according to seizure duration were statistically significant ( 2=26.074; p<0.001) and there was no death under the thirty-four seconds of seizure duration. As a result, it is though that the dose of 10 mg/kg of cloxyquin starts to be effective on epilepsy and reducing the seizure duration will also reduce deaths. KEYWORDS: Epilepsy, Pentylenetetrazole, Cloxyquin, Valproic acid, TRESK