Evaluation of the protective effects of rosmarinic acid on acute pancreatitis in rats

Aim: Acute pancreatitis is a serious cause of hospitalization, seen in 5 to 35 people per 100,000 population. Approximately 5-10% of hospitalizations result in death. Regardless of the etiology, intra-abdominal abscess, necrosis, shock and serious lung diseases occur after IL-1, IL-6, IL-8, IL-10, IL-11, TNF, PAF, NO formation in the body after pancreatitis. New studies are planned every day on the treatment of pancreatitis. The aim of our study is to examine whether rosmarinic acid can be an effective treatment for acute pancreatitis by taking advantage of its antioxidant, anti-inflammatory and other properties, and to add new findings to science on the treatment of such a serious and fatal disease. Materials and Methods: A total of 28 animals were used during the experiment, and 4 groups were formed with 7 animals in each group. Group 1 is the control group. During the experiment, the rats were injected with saline intraperitoneally and no drugs were used. The rats in group 2 were injected with 75 μg/kg Cerulein every hour intraperitoneally at one hour intervals, a total of 4 times.Group 3 experimental animals were given 50 mg/kg Rosmarinic acid with a way of peroral gavage. The rats in Group 4 were given 50 mg/kg Rosmarinic acid by peroral gavage after 75 μg/kg Cerulein was injected intraperitoneally 4 times per hour. Afterwards, all animals in the study groups were sacrificed by exsanguination, blood samples from the heart and pancreatic tissue were taken for examination. Results: In the acute pancreatitis group, tissue necrosis, edema and inflammation were observed. In addition, a statistically significant difference was found in serum TNF-α values compared to other groups.It was observed that the rosmarinic acid group did not harm the rats and tissues with both histopathological and serum values close to the control. It was determined that the use of Rosmarinic acid after acute pancreatitis had a positive effect on4 tissues and blood values, but still did not cause complete recovery. Conclusion: In the case of acute pancreatitis, it was concluded that Rosmarinic acid has a partial curative effect, but still does not provide a complete cure. Key Words: Experimental pancreatitis, Cerulein, Rosmarinic Acid