Comparison of the protective efficacy of mangiferine and amlodipine in the prevention of hepatotoxicity caused by tetracycline use in rats

Aim: Studies have shown that almost all antibiotic derived drugs have toxic effects on the liver. While these effects may be minimal, they may also lead to important problems such as chronic hepatitis, cirrhosis, and acute liver failure. In this study, we examined the protective effects of Mangiferine (MNG) and Amlodipine (AML) in the case of hepatic toxicity due to Tetracycline (TTC) use. In order to reveal which of these protective activities was superior, the groups were compared with biochemical, histological and immunohistochemical methods. Materials/Methods: In this study, were used adult 42 male Wistar Albino rats. Rats were divided into 6 groups in groups of 7. Group 1; control group, Group 2; The TCN group was given TTC peroral (p.o.) by gavage at a daily dose of 200 mg/kg for 2 weeks. Group 3; It is the AML group, and AML is administered p.o. with a daily dose of 1 mg/kg for 4 weeks given. Group 4; It is the MNG group and MNG is administered p.o. at a daily dose of 20 mg/kg for 4 weeks given. Group 5; While TCN+ AML group, Group 6; TCN+ MNG group was given drugs with the same dose and duration. Results: While the Total Antioxidant level of the TCN group was lower than the other study groups, Total oxidant level was found to be significantly higher than the other study groups. When looking at liver function tests; While the AST and ALT values of the TCN group did not differ significantly from the TCN+ AML group, they were found to be significantly higher than the other groups. As a result of H&E and PAS staining of TCN+AML group; Compared to the TCN group, it was observed that the congestion decreased, sinusoidal dilatation and pycnosis in hepatocytes continued. In the TCN+MNG group; Compared to the TCN and TCN+AML groups, a more normal hepatic structure was observed. In the TCN+ AML group, IL-6 and APAF-1 expressions were seen to be moderate compared to the TCN group, while the expressions were less intense in the TCN+ MNG group. Conclusion: As a result of our Biochemical, Immunohistochemical and Histopathological evaluations, it was observed that Mangiferin had more protective activity in case of hepatotoxicity. Keywords: Tetracycline, Amlodipine, Mangiferin, Hepatotoxicity.