Moleküler baskılanmış polimerlerle anjiyotensin-II'nin tayini

Angiotensin-II (ANGII) molecule is an important biomarker for the diagnosis and follow-up of some infections, tumors and especially cardiovascular diseases. Although the analysis of ANGII can be carried out routinely in health applications, the determination of this protein molecule from the blood is quite laborious and expensive. There is no study for the recognition of ANGII directly from blood using molecular imprinting technique which is a reliable technique for the preparation of affinity systems. In this study, ANGII-imprinted polymeric cryogels (ANGII-MIP) were designed and produced using cross-linker, functional monomer and template molecule (ANGII) at different ratios for the determination of ANGII, as an alternative to the present methods. Synthesized ANGII-MIP was characterized by surface area measurements (BET), swelling tests, and scanning electron microscopy (SEM). Surface area of ANGII-MIP was found to be 52 m2 /g, and the swelling ratio of the MIP columns was calculated as 94.3%. In order to obtain template-specific cavities in ANGII-MIP column, ANGII molecules was removed from the column with success over 80%, using 0.5 M NaCl solution. The AngII–non-imprinted (ANGII-NIP) cryogels synthesized without ANGII molecules and all studies were performed for ANGII-NIP, as well. Selectivity of ANGII-MIP was investigated using Arg8 Vasopressin, Angiotensin-I molecules. The reusability of the synthesized ANGII column was also studied in this study. Keywords: Angiotensin II, Polymeric Cryogels, Molecular Imprinting