Investigation of the role of bromelain administration in the treatment of cyclophosphamide-induced renal injury in rats

This study was carried out to investigate the effects of bromalein on cyclophosphamide (CYP)-induced oxidative stress and nephrotoxicity in rats. In our study, 42 male Wistar albino rats were used and divided into 6 groups with 7 animals in each group. Control group received a single dose of saline, BRM20 group received 20 mg/kg oral bromalein for 10 days, BRM40 group received 40 mg/kg oral bromalein for 10 days, CYP group received a single dose of 200 mg/kg cyclophosphamide, CYP+BRM20 group received a single dose of 200 mg/kg cyclophosphamide and 20 mg/kg oral bromalein for 10 days, CYP+BRM40 group received a single dose of 200 mg/kg cyclophosphamide and 40 mg/kg oral bromalein for 10 days. Cyclophosphamide was administered intraperitoneally on the 4th day of the study. Urea and creatinine levels in whole blood, malondialdehyde, protein carbonyl, 8-hydroxy deoxy guanosine, glutathione, nitric oxide and prostaglandin E2 levels, catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase activities in kidney tissue were analysed. One-way analysis of variance and Tukey's test as multiple variance analysis were used to evaluate the results. The toxic effects of cyclophosphamide were determined by the increase in oxidative stress and inflammatory parameters and decrease in glutathione and other antioxidant levels. It was observed that 20 mg/kg bromalein administration with cyclophosphamide partially improved the toxic effects of cyclophosphamide, while 40 mg/kg bromalein administration caused improvement in all oxidative stress and inflammatory parameters. In conclusion, it was determined that cyclophosphamide and bromalein administration together ameliorated the toxic effects of cyclophosphamide. Key Words: Cyclophosphamide, Kidney injury, Oxidative stress, Inflammation, Bromalein