Scorzonera hieraciifolia ve scorzonera longiana türleri üzerinde farmakognozik çalişmalar

It is very important to isolate and investigate the biological activity of naturally occurring compounds due to their potential as active pharmaceutical ingredients. Scorzonera L. species have been reported to be consumed as food and used in traditional medicine. Within the scope of the thesis study, pharmacognosic studies were carried out on Scorzonera longiana and Scorzonera hieraciifolia species grown in Turkey. The underground tubers of both plants were extracted with methanol according to the maceration method, and the crude methanol extracts were fractionated with n-hexane, dichloromethane, n-butanol and water. As a result of GC-MS analysis of n-hexane fracitons S. longiana and S. hieraciifoli, eight and eleven nonpolar compunds were identified, respectively. A total of 18 compounds, 7 New and one known dihydroisocoumarin scorzolongin I (SLD-5), scorzolongin II (SLD-6), scorzolongin III (SLD-7), scorzolongoside I (SLB-1), scorzolongoside II (SLB-2), scorzolongoside III (SLB-3), scorzolongoside IV (SLB-4), scorzolongoside V (SLB-5), one known sesquiterpene (SLD-4); two known phenyl heterosides (SLB-6-7), and 7 known triterpene/sterol compounds (SLH-1-3a-c, SLD-1-3, SLD-8 and SLD-9a-c) were isolated from the n-hexane, dichloromethane and n-butanol fractions of S. longiana plant by using various chromatographic techniques and identified by spectroscopic methods. A total of 6 compounds from the n-hexane and n-butanol fractions of S. hieraciifolia; three triterpenes (SHH-1, SHH-2 and SHH-3), a new flavone heteroside (SHB-1), carbohydrate (SHB-2) and 1-octadecene (SHB-3) were isolated by various chromatographic methods and characterized by spectroscopic methods. All compounds were isolated for the first time from S. longiana and S. hieraciifolia. The antimicrobial activities of 8 new (SLD-5-7 and SLB-1-5) and two known (SLB-6-7) compounds isolated from S. longiana were investigated. Among the SLD-5-7 compounds, Scorzolongin II was found to have the lowest MIC value (33.8 μg/mL) against E. coli, Y. pseudotuberculosis, M. smegmatis, C. Albicans and S. cerevisiae. Among SLB-1-7 compounds, Scorzolongoside IV, Scorzolongoside V and 3,4,5-trimethoxyphenyl-1-O-[6-O-β-D-apiofuranosyl]-β-D-glucopyranoside were found to have MIC values of 5 µg/mL, 15.63 µg/mL and 10.63 µg/mL against M. smegmatis, respectively, and Scorzolongoside I was found to have MIC value of 14.84 μg/mL only against M. smegmatis. Keywords: Antimicrobial Activity, Coumarin, GC-MS, Isolation, Scorzonera hieraciifolia, Scorzonera longiana