Investigation of protective effect of water extract of Turkish propolis against oxidative damage induced BY T-BHP

Propolis is collected various trees and plant by bees, due to rich containing flavonoids and phenolic it has antibacterial, antiviral, antitumor effect is a natural resinous with bee products. Oxidative stress results in the deterioration of the balance between free radical formation and the antioxidant system. Oxidative stress results in free radicals that acting on lipids, proteins, DNA, carbohydrates and enzymes, causing cell damage. Erythrocytes are a very important model for experimental oxidative damage studies because they both easy to reach and the most exposed to oxidative stress conditions due to the oxidation of hemoglobin that it contains. Tert-butyl hydroperoxide (t-BHP) is an organic peroxide that causes oxidative damage in human erythrocyte cells. The aim of this study is to evaluate the protective effect of water extract of Turkish propolis against t-BHP induced oxidative damage on the erythrocyte cells. The erythrocyte cells were isolated from the blood obtained by the ethical committee approval from health individuals. The erythrocyte package obtained was divided into four groups as control, water extract of Turkish propolis, positive control (quercetin) and negative control (t-BHP) group. Red blood cells package as experimental model was treated firstly with propolis extract. Then t-BHP was added to the cells packages. Protective effects of water extract of Turkish propolis were invastigated by malondialdehyde (MDA, final product of lipid peroxidation), total oxidant capacity (TOC), total antioxidant capacity (TAC) and antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT). In the water extract of Turkish propolis group, MDA, CAT, SOD, TOC measurements were found to be significantly lower than those in the negative control group (p<0.05); whereas TAC measurements were found to be significantly higher than those in the negative control group (p<0.05). The protective effect of water extract of Turkish propolis against oxidative damage induced by t-BHP on erythrocytes was revealed. Decreased SOD and CAT activities may be explained by water extract of Turkish propolis extract have antioxidant compounds that they removes free radicals from the cells.