Real-time pcr techniques for detection Mycoplasma gallisepticum in chicken trachea and statistical analysis

All over the world and in Turkey the poultry industry in our country and causing serious economic losses in chickens as a common chronic respiratory disease (CRD) caused by Mycoplasma gallisepticum's chicken trachea Real-time PCR method was accurate by and rapidly diagnosesed. Real-time PCR of Mycoplasma gallisepticum pure molecular level detection limit of 10 CFU / ml less than the detection limit and the samples were artificially contaminated with 1.0x103 CFU / ml respectively.Live chickens received 110 units (5 swabs per sample) swab tracheal samples were analyzed. Microbiological methods, 8 (7.2%) swab case of Mycoplasma spp. isolation was performed. Real-time PCR method was detected 32 (29.9%) samples of Mycoplasma gallisepticum DNA . Mycoplasma gallisepticum DNA levels in the study 6.39x104 copies/ml and 2 copies/ml was found to change. Mycoplasma gallisepticum genomic DNA copy numbers of these examples is an important relationship between crossing point values were tested with linear regression analysis (R2 = 96.6%, p <0.01). These results suggest that the determination of the real-time PCR of Mycoplasma gallisepticum infected flocks, and routine studies to determine the severity of infection a rapid (40 to 45 minutes) and that it was concluded that a reliable method.Key Words: Chicken, Mycoplasma gallisepticum, mgc2 gene, Real-time PCR,Linear Regression Analysis