Production and characterzation of alkaline, thermophilic, highly thermostabil and oxsidant resistant cellulase from a thermophilic Bacillus sp.

In this study, Bacillus strains isolated from the samples of soil, natural fertilizer and biocompost in Adana. The optimum temperature and pH for strains growth and enzyme production were determined on CMC-plate. After that the partial purified enzyme was characterized and the industrial using of enzyme was researched.Using SDS-PAGE two bands is found with molecular mass 80 kDa and 72 kDa, respectively. The partially purified enzyme showed optimally activate at pH 9.0 and 100 °C and it was stable from pH 6 to 13 with more than %65 activity. And it was stable from 20-120 °C with more than 83% activity after incubating at 100 °C for 60 min. The enzyme was inhibited in the presence of EDTA, MnCl2, ZnCl2, MgCl2, SDS, Triton-X-100, Tween 20, Tween 80, Mercaptoethanol, Phenontroline, PMSF, Iadoasetamide and urea up to 34, 53, 29, 36, 17, 32, 31, 29, 25, 33, 13, 34, and 30 respectively. However, it was increased in the presence of CaCl2, CoCl2 and H2O2 (31, 21 and 16 %, respectively). The activity was stable in different NaCl concentration from 3 to 30 % with more than 66 °C activity. Highly thermostability, pH stability, and stability in the presence of detergents, metal ions, and some additives (for example H2O2) make this enzyme potentially useful in textil, laundry and in other industrial applications.