Investigation Teucrium parviflorum and Teucrium polium plants in terms of chemical and biological activity

Aim: It was aimed to determine that the total phenolic and flavonoid amounts and antioxidant-anticholinesterase activities of the ethanol extracts of T. parviflorum and T. polium plants that are cited as their antioxidant activity in the literature, also phenolic contents were studied with LC-MS/MS, and volatile oil contents were studied with GC-MS. Material and Method: Root and aerial parts of T. parviflorum and T. polium were extracted by ethanol and, total phenolic and flavonoid amounts of these extracts were detected. Antioxidant activities were determined by DPPH, ABTS, metal binding and CUPRAC methods. Also, anticholinesterase activities were determined via Ellman method. Volatile oils obtained by Clavenger method were analyzed by GC-MS. In addition, phenolic compound contents were obtained with LC-MS/MS. Results: T. polium roots were found that they include the highest amounts of total phenolic and flavonoid contents. None of the extracts have demonstrated the metal binding and acetylcholinesterase inhibition activity. Activities of root and aerial parts extracts of T. polium have shown better antioxidant potential than T. parviflorum for all methods. Also, it was determined that root extracts of T. polium shows better activity than BHT used as standard for DPPH; and than BHA compound which are used as standard for ABTS methods. Major compounds of volatile oil contents obtained by GC-MS analysis were gained as germakren D (32.7%) for T. parviflorum; and (Z)-β-farnesen (28.9%) for T. polium. Also major compounds of phenolic contents obtained by LC-MS/MS analysis were gained as naringenin (16327 g/g extract) for T. polium aerial parts, (-)-epigallocatechin gallate (3694 g/g extract) for T. polium roots, and hesperidin (5687 g/g extract) for T. parviflorum aerial parts. Conclusion: T. polium might be considered as having high antioxidant potential due to its phenolic content. Chemical contents with LC-MS/MS and anticholinesterase activity of T. parviflorum plant was studied for the first time.