Study of plasma polymeri̇zati̇on techni̇que on ti̇tani̇um alloys bi̇omateri̇al for i̇mmobi̇li̇zati̇on wi̇th bone morhogeneti̇k protei̇n

Titanium is the material of choice in the production of implants used in orthopedics, spine surgery and dentistry due to its excellent mechanical properties, chemical stability and biocompatibility. The loose interaction between the implants and bone result in implant loosening, pull-out and screw breakage. Serious symptoms and probable revision surgeries can develop because of implant related problems. The acceleration of new bone development, production of new implant types and use in health area are strategically important steps. Therefore, waist and spine surgery in commonly used method, the bone tissue and the interaction (osteointegration) to maximize the level of bone tissue formation bone proteins which induce morphogenetic protein (rhBMP-2) is placed on the bone and then the titanium alloy implant material (transpedicular screws) is not fixed. However, completely natural (and therefore biocompatible) this protein spine pouring powdered, both increases the costs and due to dose excess of bone still complications (heterotopic ossification, osteolysis, inflammatory cyst formation, etc.).In the millenium, dynamic stabilisation and percutaneous instrumentation techniques have been started to be widely used. Interbody fusion is used in minimally invasive percutaneous spine surgery while no bony fusion was performed in dynamic stabilisation. New bone formation around the screw minimizes the risks of screw loosening, pull-out and screw breakage. In our country, only hydroxyapatite (HA) coated screws are available for increasing the screw bone integration. HA coating is expensive and cannot be performed in our country. Since HA coating is dependent on European countries, we planned to coat the spinal implants with recombinant bone morphogenetic protein (rhBMP) by plasma polymerisation technique. In order to solve this problem in the research of material surface by plasma polymerization techniques in the creation of appropriate chemical groups and continue the bone morphogenetic protein (rhBMP-2) approach is used directly on the implant immobilization. In the plasma polymerization technique, the original monomer (cysteamine) and load discharge type (radio frequency) is used. Furthermore, the method of processing parameters (power (W) time (min), monomer flow rate (ml / min) by changing the surface physical and chemical differences occurred were identified by various characterization techniques. The results are evaluated in forming nanoscale film flow at the dynamic environment stable plasma polymerization parameters were selected. Optimal parameter power by resolution: 30 W Time: 9 min flow rate: 10 ml / min . The modification by plasma polymerization technique to the selected processing conditions and after the activation (2.5% v / v, glutaraldehyde) step continued in is 2.5% mg/ml rhBMP-2 arrested for 2 hours. It is proven by histological testing the implant surface rhBMP-2 enhances bone tissue formation with the immobiliziton. As a result, spinal implants plasma polymerization coating technique with bone formation by increasing the bone morphogenetic protein (rhBMP-2) approach , outside the body (in vitro) / and animal (in vivo) has been tested and evaluated.