Preparation of different pharmaceutical dosage forms containing transforming growth factor and investigation of their activities based on cell culture

Objective: Within the scope of the thesis; It is aimed to prepare different pharmaceutical forms using TGF-β1 protein and to demonstrate their efficacy and contribution to protein stability in cells involved in wound healing mechanism. Materials and Method: Three different delivery systems have been developed for TGF-β1 protein. These formulations are PLGA nanoparticles, PVA/Kitozan hydrogels and PVA/PAMAM hydrogels. PLGA nanoparticles were obtained by double emulsion solvent evaporation method. PVA/Kitozan and PVA/PAMAM hydrogels are prepared by the freeze-thaw method, which is the physical gelling method. Results and Conclusion: Characterization studies of prepared polymeric drug delivery systems have been carried out. It was then tested on NIH-3T3 and HaCaT cell lines and was found to increase cell viability and proliferation, as well as protein stability, especially when compared to free protein. Migration has been done with optimized formulations and it has been observed to increase. Characterization analyses were carried out by creating combination systems among the formulations developed and the comparison of the obtained results was investigated based on cells.