Investigation of the biological activity of Xanthoparmelia pulla species distributed in Turkey.

This study aims to investigate the antioxidant activities and cytotoxic effects of methanol extracts obtained from Xanthoparmelia pulla (Ach.) O. Blanco, A. Crespo, Elix, D. Hawksw. & Lumbsch, a speciesdistributed in Turkey. The amounts of secondary metabolites for Xanthoparmelia pulla were determined using High-Performance Liquid Chromatography. According to the obtained results, the highest amount was found to be Salazinicacid (37.23 ± 9.21 mg/g extract). The total phenolic content (172.77 ± 10.50 mg GAE/g extract) and total flavonoid content (9.25 ± 1.32 mg CA/g extract) of Xanthoparmelia pulla extract were determined. DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] methods were used for antioxidant activity evaluation. It was determined that Xanthoparmelia pulla extract had the highest DPPH scavenging activity, showing 87.41% inhibition at a concentration of 2 mg/mL. Similarly, thehighest ABTS scavenging activity was found to be equivalentto 2.29 ± 0.31 mmol/L Trolox at a concentration of 0.5 mg/mL. Cytotoxic activity assessment was performed on MCF-7 and MDA-MB-231 usingthe MTT assay. According to the results, Xanthoparmelia pulla extract exhibited 33.16% viability at a concentration of 125 μg/mL in the MCF-7 cellline, while 60.58% viability was observed at a concentration of 250 μg/mL in the MDA-MB-231 cell line.