Ghrelin gene polymorphism in vitamin B12 deficiency

Vitamin B12 is a vitamin which can not to synthesized from the human organism. Mainly it?s required for the synthesis of DNA (deoxyribonucleic acid) and myelin. The incidance of vitamin B12 defiency is varable from society to society in different groups, socioeconomic level and eating habits. When the community realised vitamin B12 defiency incidance is %3 to %40, B12 interest is increased. The world?s poorest regions, particularly in rural regions childern?s from malnutrion the frequency of vitamin B12 defiency is known to be as high as 22-66%. 7-17 age group of 960 children in Turkey, it was identificated % 5.9.With this study ghrelin values and ghrelin gene polymorphism are evaluated in vitamin B12 deficient patients. In same dietary equal family some members shows signs of vitamin B12 deficiency some does not. So this may be the cause of ghrelin gene polymorphism. Before vitb12 deficiency occurs, Vitamin B12 supp and getting necessary precoutions are aimed.In different studies using DNA sequencing many different uninucleotide polymorphism are identified in ghrelin gene. Most impant ones are promotor -501 A/C, Arg51Gln, Leu72Met and Gln90Leu.In our study 27 female and 30 male vitamin B12 deficient 42 patients, 19 female and 23 male 42 healthy controls are evaluated. Achyl ghrelin values are found 14.5±10.0 pg/ml in patient group and 24.2±14.2 pg/ml in control group achyl ghrelin values are found statistically significant between patient and control group (p<0.05). Deachyl ghrelin is found 242.3±206 pg/ml in patient group and 394.0± 170 pg/ml in control group. Deachyl ghrelin values are found statistically significant between patient and control group (p<0.05). In patient and control group -501 A/C polymorphism are studied. Statisticaly -501 A/C polymorphism in ghrelin gene as CC genotype is found increased frequency in patient group (p<0.05). For promotor -501 A/C polymorphism allele frequency is not found significant (p>0.05). For Gln90Leu polymorphism in group heterozygot Gln/Leu genotype is found in increased frequency (p<0.05). For Gln90leu polymorphism allele frequencies are not found significant in control group (p>0.05). For Arg51Gln and Leu72Met polymorphisms when genotype and allele frequencies are compared no significant differency is found (p>0.05). Statistically significant in patients with vitamin B12 levels 200 pg/ml under.We think ghrelin gene may have an impant role in Vitamin B12 deficiency?s immunogenetics. We believe new studies should be done in Turkish population or in other populations to determine whether other polymorphisms provide supp for the disease or not.We think increased promoter -501 and heterozygot Gln/Leu varient frequency in ghrelin gene plays an impant role in Vitamin B12 deficiency etiopathogenesis.Keywords: vitamin B12 deficiency, ghrelin, gene polymorphism