The detection of the total antioxidant capacities of new benzothiophen derivatives and their effects on GSTM2 isozyme

Benzothiophenes have begun to form the subject of projects that aim to test their potential to be used as a drug, recently. In the first part of this study, antioxidant capacities of two benzothiophene derivatives, whose not being subjected in another project was detected by Reaxys®, were determined by three different methods: ABTS, DPPH and Galvanoxil. In the second step, the possible inhibition / activation effects of these derivatives on GSTM2-2 isozyme were investigated. Both of the benzothiophene derivatives showed very low antioxidant capacities compared to the standard antioxidants tested. As a result of the ABTS procedure for derivative-1, the EC50 value was calculated as 42.52 μM. However, in the experiments performed for the other two methods, the % RSA values were not high enough to calculate the EC50. The EC50 value could not be reached in any of the tests on Derivative-2. The value of Vmax for derivative-1 was reduced but the Km value was not changed. SigmaPlot software predicted the full noncompetitive inhibition model. Derivative-2 showed activation at low concentrations but inhibition over 5μM final concentration. The software stated this effect to be either full competitive or full noncompetitive inhibition; a possible mixed-type model. Keywords: ABTS, Benzothiophen, DPPH, Galvinoxyl, GSTM2, Inhibition model