Paraoxonase-1 gene in patients with acute ischemic stroke disease investigation Q192R ve L55M polymorphisms

The effect of increased oxidative stress in development of acute ischemic stroke is well known. One of the antioxidative systems against oxidative stress in human body is paraoxonase (PON) enzyme that protects low density lipoproteins (LDL) against oxidation. In this study we aimed to search the on PON1 gene polymorphisms, Q192R and L55M in patients with ischemic stroke.The DNAs extraction was obtained from blood samples of the 50 patients pastly diagnosed with and 50 participants of control group who were presented to emergency clinic. Genotypes were obtained with polymerase chain reaction (PCR) and AIw I and Hsp92II restriction enzymes were used for Q192R and L55M polymorphisms, respectively. Analysis of data was done with Chi-Squire and Fisher?s exact tests.A statistically significant difference regarding Q192R polymorphism was found between ischemic stroke patients and control group (P = 0.05). There was no statistically significant difference about L55M polymorphisms in patient and control groups (P>0.05).In our study, we observed PON-1 gen Q192R and L55M polimorfizm in patient with ischemic serebrovascüler disease. Consequently, we suggest that PON-1 gen Q192R polimorfizm contribute patogenesis of serebrovasculerdiseas. We believe that more studies are needed in this subject.Key Words: İschemic stroke, paraoxonase, polymorphism