The identification of indicator bacteria and determination of their antibiotic sensitivity in laying hens in Aydin province

Poultry frequently encountered to Escherichia coli and Enterococcus spp. infections all around the world. Before the recent prohibition of on the antibiotic, feed additives as a growth promoter have been widely used in poultry industry. However, the development of antibiotic resistance problem and the public concern have lead to a complete prohibition on the dietary. It is necessary to well define the current status of antibiotic resistant concerning the isolates of Escherichia coli (APEC) and Enterococcus spp. Thus, it would be beneficial to determinate the efficient therapeutic antibiotic so as to cure the disease induced by infection. The scope of this study is the isolation of E. coli, E. faecium, E fecalis and identification of the potential antibiotic resistance against Penicillin-novobiocin, Tetracycline, Enrofloxacin, Trimethoprim-sulfamethoxazol, Florfenicol, Amoxicillin Clavulanic acid, Gentamicin and Kanamycin are the scope of this study. In this study, 21 (10 %) E. fecalis, 24 (12 %) E. faecium and 40 (20 %) E. coli isolation and identification were made from a total of 200 cloacal samples which were collected from hen laying poultry houses, upon biochemical properties and carbonhydrate fermentation tests. According to antibiogram test results it was revealed that, E. fecalis was highly resistant to tetracyclin and kanamycin, E faecium was was highly resistant to kanamycin and E. coli was was highly resistant to penicilin novobiocin. Besides, it was revealed that E. fecalis was totally susceptible to penicilin novobiocin, amoxycillin clavulanic acid and gentamicin, E. faecium was totally susceptible to penicilin novobiocin and amoxycillin clavulanic acid, E. coli isolates were totally susceptible to florfenicol and gentamicin.