Molecular and bioinformatic characterization of 16s rDNA regions in some Bacillus isolates

horpological and biochemical data must be supported by molecular data when organısms are classified. Two organisms may share simillar features, due to also such as lateral gene transferring and convergent evolution events, when organisms are classified. Therefore morphological and biochemical data usually aren?t sufficent and reliable in classifing organisms. Thus, supporting morphological and biochemical data by molecular data for true classification is very crucial. Molecular approachs are become very useful in particular bacterial taxonomy. İmportance of molecular approachs are better understood when existence of bacteria which majority of bacterial diversiry in nature are thought and uncultivated (fastidious) are considered. Also recently are often appylied those molecular approachs such as 16S rDNA where are become sometimes insufficent fenotipic approachs in clinical diagnosis and identification. 16S rDNA sequencing analysis is one of the most frequently used methods. Recently, although some protein genes also are used, due to 16S rRNA (rDNA) gene is present universal among bacteria, It has been indispensable for bacterial taxonomy.In our study, we evaluated that Bacillus isolate which has been identified in genius and species according to morphological and biochemical method by sequencing analysis of 16S rDNA regions.Sequencing results of 16S rDNA regions showed 99?100% homology with known sequence data in gene bank. This results confirmed species levels of eight isolates which identified as Bacillus megaterium, Paenibacillus polymyxa, Bacillus thuringiensis and the remaining five B. Subtilis according to phenotypic data. Furthermore, phylogenetic relations among this isolates also was determined using this data.Key Words: 16S rDNA, PCR, Bacillus, Lateral gene transferring, Convergent evolution