Investigation of antioxidant system indicators in rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to Bisphenol F

Bisphenol F (BPF) is a bisphenol A analog that is found in many consumer products used in daily life. In the present study, primary cultured hepatocytes of the rainbow trout (Oncorhynchus mykiss) were exposed to 7 different concentrations (0, 15.63, 31.25, 62.50, 125, 250, and 500 µM) of BPF for 24 h. Cellular damage by BPF was determined using the lactate dehydrogenase cytotoxicity test. Next, antioxidant defense system indicators were measured. According to the results, the cellular damage to the hepatocytes was in relation to the increase in the concentration of BPF. Superoxide dismutase activity was determined to increase significantly in all of the groups treated with BPF. Catalase activity decreased with all of the concentrations of BPF. The reduced glutathione levels increased with concentrations of 15.63, 31.25, 62.50, 125, and 250 µM of BPF, whereas they decreased with 500 µM of BPF. The glutathione peroxidase activity remained unchanged with 15.63 µM of BPF; however, statistically significant increases were determined in the other groups. Glutathione-S-transferase activity was observed to increase only with 125 µM of BPF, and remained unchanged in the other groups. The results showed that BPF leads to cellular damage by disrupting the antioxidant balance in the hepatocytes of fish. Keywords: Antioxidant system indicators, Bisphenol F, Hepatocyte culture, Rainbow trout (Oncorhynchus mykiss)