CD70/CD27 molekülleri ile ilişkli mekanizmaların kolorektal kanserli hastalarda incelenmesi

Colorectal Cancer is among the highest death-causing cancer types around the world. Immune checkpoint molecules, such as CD70, promise a better understanding of tumor microenvironment in colorectal cancer. CD70 is a costimulatory factor present on B and T-cells. Binding of CD27 to its ligand results in evasion of tumor cells from the immune system. We aim to explore the expression profile of CD70, CD27, CD3, and FOXP3 molecules in tumor microenvironment (TME) of CRC patients and to detect the recruitment of tumor-infiltrating lymphocytes (TILs) present in TME. Also, we aim to investigate the predictive diagnosing value of soluble-CD27 for monitoring cancer. The expression profile was analyzed by western blot wet transfer system. Soluble CD27 protein level in patients' serum was detected by ELISA. We detected the low expression of CD70 (15.15%) in TME of CRC samples however, high abundance of CD27 (84.85%) was detected. Our results revealed that there is high recruitment of CD3+ lymphocytes (78.79%) and FOXP3+ Tregs (48.49%) in TME. Additionally, sCD27 serum level was found to be significantly high (p<0.0001.) in patients' sera and there is a strong correlation between high serum level of sCD27 and CD70 positivity and CD27 negativity in TME. Although no correlation was found between patients' clinical outcome and sCD27 serum level when distant organ metastasis present sCD27 level was significantly high (p=0.045). Consequently, CRC is not one of the tumors that are strongly express CD70 in TME however, sCD27 could be considered as a good serum biomarker.