Molecular mechanisms of pyrazinamide resistance among strains of mycobacterium tuberculosis clinical isolates in Cukurova region

Tuberculosis (TB) is one of the oldest diseases. Today, the increasing number of patients infected with multidrug-resistant (MDR-MDR) and common drug-resistant (XDR-YID). Tuberculosis bacilli is the biggest problem in the control of the disease. In this study, 100 M. tuberculosis complex strains clinical materials isolated from patients with a history of pulmonary tuberculosis or suspected contact in 8 provinces and Tuberculosis Dispensary affiliated to Ministry of Public Health Institution Adana Regional Tuberculosis Laboratory. in the 100 M.tuberculosis complex strains, 97 were M. tuberculosis (97%), 3 were M. bovis (3%), as a result of the antibiotic susceptibility test, 59 of the isolates were PZA resistant (59%) and 41 of them were PZA sensitive (41%). As a result of DNA sequence analysis of genes responsible for PZA resistance, 3 PZA natural resistant M.bovis, in 56 of the remaining 49 PZA resistant isolates, pncA (88%) and 9 isolates had panD (16%) mutations, 41 PZA sensitive isolates, 8 pncA (20%) and 5 panD (12%) mutations has been observed. It was observed that 3 PZA resistant and 1 PZA sensitive isolates had both pncA and panD mutation. Keywords: Mycobacterium tuberculosis, drug resistance, Pyrazinamide, pncA gene, panD gene