Effect of mad honey (Grayanotoxin) on acute and chronicle pain beviour

Introduction: Aim of this study is to investigate whether mad honey can be used to reduce pain in acute and chronicle pain (diabetic neuropathic pain) as used as pain killer in alternative medicine.Material and Methods: This study was made as an animal experiment. For acute pain, normoglycemic mice; for neuropathic pain, mice having diabetes mellitus by giving streptozocine were used. After making seven groups of mice, their latencies were measured with plantar analgesymeter. Latency of mice with diabetic neuropathy was measured again after being diabetic neuropathy and their latencies were saved to data. At the final stage of study, mice treated with grayanotoxin were measured for their latencies for different doses and times of giving GTX.Findings: In the study for acute pain, GTX given 3 different doses increased latencies statistically significantly (P<0.05). After GTX given 0.1 mg/kg latency was 3.5±0.7 sn at the 10th minute and was 3.9± 0.5sn at the 20th minute. Acute effect of GTX started immediately at the 10th minute and continued to 100th minute, this showed us the period of effect.. Before GTX the latency of a group was 2.5±0.7 sn, after given 0.1 mg/kg GTX latency was 3.5±0.7 sn at the 10th minute, and 3.9±0.5 sn at the 20th minute, this was statiscally significant (p<0.001). Latencies of mice given 0.1 mg/kg GTX was about two times at the 20th minute. Latency was 3.2 ± 0.7 sn at the 30th minute and 3.3±0.6 sn at the 60th minute, this was statistically significant. Latency in mice given 0.2 mg/kg GTX i.p. was 2.4±0.4 sn before GTX, was 3.1±0.6sn (p<0.01) at the 10th minute, 3.0±0.3sn (p<0.05) at the 20th minute, both of them was statistically significant.In neuropathic pain study, GTX made no difference in three different doses first, but decreased latencies significiantly at the 60th and 100th minutes (p<0.05).Latencies in neuropathic mice were similar to normal after given GTX(p<0.05). Before GTX given 0.1 mg/kg latency was 3.2±0.6 sn, after GTX latency was 3.0±0.9 sn at the 10th minute, 3.2±0.6 sn at the 20th minute, 3.4±0.6 sn at the 30th minute, 2.6±0.5sn at the 60th minute, 2.4±0.6 sn at the 100th minute (p<0.05 sn). These were similar to before being neuropathic in mice. Latency in mice given 0.2 mg/kg GTX i.p. was 3.2±0.4 sn before GTX, 2.6±0.8 sn at the 60th minute, 2.4±0.5 sn at the 100th minute (p<0.05). In this group latencies were similar to onset values before neuropathy as the other group (p<0.05).Conclusion: In neuropathic pain decrease of latencies after given GTX showed that GTX can be used for treatment diabetic neuropathy. Study of long term giving GTX to neuropathic mice can evaluate the accuracy of these data. In normoglysemic mice GTX increased the latency from first minutes and made mice to feel the pain late. Mad honey(grayanotoxin) which is used for pain killer in alternative medicine can be used in modern medicine supported with further laboratory and clinically studies.