Experimental diabetes in rats by the relationships between some acute phase proteins and trace elements

Diabetes mellitus, characterizing with increased blood sugar, is a chronic and dangerous disease. Diabetes mellitus commonly exists all around the world. Slowly but continuously destruction occurs at patients with diabetes mellitus. Acute phase response is a nonspecific reaction that appears a little while after tissue damages. Infections, immunologic and neoplastic diseases, parasites or trauma can cause the acute phase response. Releasing acute phase proteins from liver such as CRP, ceruloplasmin and haptoglobin is the most characteristic feature of acute phase response. In this study, determining levels of acute phase proteins such as CRP, haptoglobin, ceruloplasmin and some of important trace elements related to acute phase proteins such as Fe, Cu and Zn; and searching a relationship between their levels, was purposed.27 Wistar rats were used in this study. 50mg/kg streptososin(572201, Calbiochem) in 0.01 M sodium citrate tampon(pH 4.5) was injected intraperitoneally to 16 rats for developing diabetes. 21 days later following injection, rats were left to hunger for 12 hours. After determination of blood sugar level, intracardiac blood samples were taken under ether anesthesia; and euthanasia was applied with cervical dislocation. Following the blood take, serum samples were distinguished and CRP, haptoglobin, ceruloplasmin, Fe, Cu, Zn analyses were performed. Results were statically assessed. In the rats with experimental diabetes mellitus, it was observed that CRP and haptoglobin levels were significantly affected but there were no difference in ceruloplasmin levels between control and diabetes groups. It was found that in diabetes group Fe and Cu levels were higher than control group; however Zn level was lower.As a result; determining levels of acute phase proteins such as CRP and haptoglobin and some of trace elements such as Fe, Cu, Zn can be accepted as useful parameters for observation of inflammatory process, detection of disease complications and controlling of treatment efficiency.Key words: Diabetes, acute phase proteins, trace elements, rat.