Determination of genetic diversity of Astragalus L. species distributed in erzi̇ncan and its surroundings by molecular methods

In this study, Astragalus L. samples were collected from 42 locations of Erzincan determined in the vegetation period of 2021. As a result of the morphological studies, 12 different species were determined. 3 of them are endemic (Astragalus pennatulus, Astragalus karamasicus, Astragalus lycius). The genetic relationship between the identified species was investigated with ISSR and IRAP molecular markers. The 5 studied IRAP primers (3LTR-5, 5LTR1, LTR 6150, NIKITA E2647-, SUKKULA) yielded bands of 100-700 bp. Polymorphism was calculated as 90% according to IRAP primers. 16 ISSR primers (UBC868, UBC817, UBC807, UBC812, UBC816, UBC825, UBC824, UBC830, UBC836, UBC840, UBC848, UBC855, UBS856, UBC841, UBC842, UBC61) were used in the ISSR method. According to the PCR results, the primers studied gave bands of 100-2000 bp in length. Polymorphism was calculated as 90.8% according to ISSR primers. Dendrograms were created using the ISSR and IRAP data and the UPGMA method. These studied techniques turned out to be suitable markers for characterizing Astragalus species.