Investigation of some pollen grain features of blood oranges and determination of hybrids in Clementine × Blood orange crosses with SRAP markers

`Clementine? mandarin (Citrus reticulata `clementine?) between with different genotypes of blood oranges (Citrus sinensis Osbeck) has been realized hybridization in 2010 and 2011 years. `Clementine? was used as female parent and `Moro? (M), `Sanguinello? (S), `Tarocco? (T), A1, A2, A3, H1, H2, H3, K1, K2 cultivars and local genotypes as male parent was used in total 11 parents. Before hybridization, because of parent blood oranges species at 11 varieties for 2011 pollens applied anther count, pollen count, viability and germination test. Three varieties together (M, S, T) was done on viability test as P<0.05 significance 22.15% S variety, eight genotypes was done together on test A3 genotype with %44.15 was found high from others. As germination test, the highest germination was obtained at H3 genotype on %1 agar+ %25 sucrose media. Anther count for each flower average is 22.2 a number value was `Tarocco? , on hemasitometric lam total pollen count of one flower was found Sanguinello variety as high. At hybridization results 1397 flower was crossed and was obtained 27 fruit, 86 seed for 2010. 580 flower was crossed and was obtained 43 fruit, 339 seed for 2011. With obtained 135 seed for H3 and 142 seed for A3 as male parent is promising for future about pollination feature. Seeds of 2010 and 2011 fruits was taken and planted into cotainers, was obtained and was growed hybrid seedlings in a greenhouse and was obtained respectively 42 and 161 hybrid plant. 13 hybrids and 2 parents (C, K1) genotypes of 2010 year was done the analysis and obtained similarity, diversity between male, female, hybrids by using SRAP moleculer markers. In total 15 genotipe was obtained 66 bands taht of which 24 bands were polymorphic. Polymorphism ratiowas 36.4%.