Investigation of self incompatibility mechanism by using cDNA AFLP in clementine (Citrus clementina hort. ex tan)

In this study pollen-pistil interaction was investigated to understand which genes are involved in self-incompatibility mechanism of Clementine mandarins. Pollen tube growth of a Clementine variety, `Algerian Tangerine Ranch Selection? (self-incompatible) was analyzed by using complementary DNA-amplified fragment length polymorphism technique to identify genes putatively involved self-incompatibility. The transcript profiles of unpollinated and self-pollinated (for 1, 2, 3, 4, 5, 6, 7th days) styles and stigmas of the Algerian Tangerine cultivar were compared. DNA profiles showing transcriptional differences between the days of pollination were excised from the gels and directly sequenced. DNA sequencens were investigated by doing BLAST analysis on NCBI database. Some unigenes were determined from the results of directly DNA sequencing. Selected gene tags showed transcriptional differences between unpollinated and self-pollinated styles and stigmas during pollen germination and pollen tube elongation.