Investigation of the effect of fulvic acid in the stomach and intestinal mucosa on the damage of the chronic water avoid stress

Aim: The objectives of this study was to investigatethe antioxidant and antiulcerogenic effects of fulvic acid (FA) on oxidative damage caused by the water avoidance stressin rats stomach and intestinal mucosa. Material and Methods: 3 experimental groups (n=6) were established (18 Sprague-Dawley rats, 300 gr): Control (C),Chronic Stres (CS) and Chronic Stres+Fulvic Acid (CS+FA). After water avoidance stress, immediately a single dose of FA (150 mg/kg i.p) was administered for 10 days to CS+FA. Tissues are histologically stained with H&E, PAS, Toluidin blue and immunohistochemical marking was applied for eNOS and iNOS. Also Total Antioxidant Status (TAS), Total Oxidant Status (TOS) , Superoxide dismutase (SOD), Glutathione peroxidase (GPx) and Catalase (CAT) levels were analyzed as biochemically of stomach. Results and Conclusions: Spalling on stomach epithel cell, extensive openings and polymorphonuclear cell migrations were observed in CS. Columnar cells was flatten and lost mucosa were observedin duodenum CS. When compare with C and CS, marking of iNOS and eNOS was showed strong reaction in CS. According to other groups OSI, highest and significantly increase of ratio in CS was observed. SOD, GPx, CAT was increased of significantly increase of ratio in CS+FA according to CS and in the light of these parameters water avoidance stress for 10 days caused of oxidative stress so cell and barrier damage altered tight junction expression and FA prevents this damage as possible antioxidant effects. Keywords: Antioxidant, Fulvic Acid, Inflammation, Oxidative Stress, Water Avoidance Stress