Research of Leishmania infantum in asymptomatic dogs where canin Leishmaniasis endemic area is Aydın/Kuşadası

The aim of this study is researching of L. İnfantum in healty dogs in Aydın/Kuşadası. In the study, 100 apparently healthy dogs were evaluated clinically in general. Each of the serum samples of animals were applied Indirect Fluorescent Antibody Test (IFAT) to determine anti-L. İnfantum antibodies and each animals peripheral blood samples taken into tubes containing EDTA were isolated DNA and real-time PCR test was applied with specific primers and probe of L. infantum. From examined 100 dogs to L. infantum detection, 23 (23%) were determined to be seropositive by IFAT method, none of the dogs DNA?s were found positive with real-time PCR test. In detected seropositive dogs contain 9 (9/44 %20,45) female, 14(14/56 %25) male; 11 (11/55 %20) between 1-3 age, 12 (12/45 %26,6) between 4-12 age; 2 (2/22 % 9,09) pet dog, 21 (21/78 %26,92) guard; 3 (3/16 %19,75) short hairy, 6 (6/40 %15) long hairy, 14 (14/ 44 %31,82) normal hairy; 15(15/60 %25) are live in garden day-night, 2(2/10 %20) are live in street day-night, 1 (1/7 %14,29) is day garden- night home, 1 (1/2 %50) is day street-night home. There was not statistically significant difference among seropositivity and age, breed, gender, hair length, place (p>0,05). As a result, according to data in Aydın/Kuşadası, where canin leishmaniasis is endemic, infected by L. İnfantum asemptomatic dogs are may be a risk factor for the people and sensitive animals.Key Words: Leishmania infantum; Asymptomatic; Dog; Seroprevalence; PCR.