Investigetion of the relationship between insulin resistance and serum asprosin and trimethylamine oxide levels in patients with polycystic ovary syndrome using metformin

PCOS is a very common hormonal disorder in women of reproductive age. Insulin resistance is a complex cellular disorder that affects many organ systems and causes serious metabolic defects. Asprosin is a proteinaceous hormone that regulates glucose hemostasis secreted from white adipose tissue, in the absence of which causes a metabolic disorder with low insulin levels. TMAO is another molecule formed as a result of the metabolism of fatty foods in the intestines and associated with insulin resistance. TMAO is another molecule formed as a result of the metabolism of fatty foods in the intestines and associated with insulin resistance. Our study consisted of 60 PCOS patients, 30 of whom were using metformin, 30 of whom were not using metformin, and 30 healthy control groups, aged 18-40 years, without chronic disease. The patients' HbA1c, AST, ALT, urea, creatinine, Lipid levels, fasting insulin level, estradiol, testosterone, CRP, TSH, FSH, LH, DHEA-SO4, CBC results were evaluated. Age, gender, height and weight values of the individuals participating in the study were recorded. Body mass indexes and HOMA-IR, HOMA Q, HOMA B values were calculated. In addition, blood was drawn into tubes with aprotin to study Asprosin and TMAO levels. There was a significant difference between the groups in terms of weight and BMI (p<0.001). It was observed that the BMI and weight values of the group taking metformin were higher than the other groups. There was no significant difference between the groups in terms of age (p=0.556), height (p=0.285) and smoking status (p=0.685). It was observed that there was a significant difference between the groups in terms of triglycerides (p=0.021). ). It was determined that this difference was caused by the difference between the metformin group and the control group, and the triglyceride level of the metformin group was higher than the control group. There was no significant difference between the groups in terms of total cholesterol (p=0.151), LDL (p=0.065) and HDL (p=0.322). There was a significant difference between the groups in terms of glucose (p=0.022) ). It was determined that this difference was caused by the difference between the metformin group and the control group, and the glucose level of the metformin group was higher than that of the control group. There was a significant difference between the groups in terms of ALT (p=0.001). It was determined that this difference was caused by the difference between the control group and the other two groups, and the ALT level of the control group was lower than the other two groups. There was a significant difference between the groups in terms of fasting insulin (p<0.001). It was determined that this difference was caused by the difference between the control group and the other two groups, and the fasting insulin level of the control group was lower than the other two groups. There was a significant difference between the groups in terms of testosterone (p<0.001). Testosterone levels were found to be higher in the group that did not take metformin. There was a significant difference between the groups in terms of HOMA-IR (p<0.001) and HOMA-Beta (p=0.008) values. It was determined that this difference was caused by the difference between the control group and the other two groups for both values, and the HOMA-IR and HOMA-Beta levels of the control group were lower than the other two groups. There was a significant difference between the groups in terms of HOMA-Q value (p<0.001). It was determined that this difference was caused by the difference between the control group and the other two groups, and the HOMA-Q level of the control group was higher than the other two groups. When the metformin group was evaluated, there was a positive correlation between TMAO and asprosin and HOMA-Q. A negative significant correlation was found between TMAO and fasting insulin and HOMA-IR. When the group not taking metformin was evaluated, there was a positive correlation between TMAO and asprosin, a significant negative correlation was found between TMAO and glucose. A significant negative correlation was found between asprosin and glucose. As a result, we found low levels of Asprosin and TMAO in our patients with high insulin resistance, and we think that new multicenter studies with a larger population are needed in order to use these two biomolecules as a biomarker. Key Words: Polycystic ovary syndrome, insulin resistance, trimethyl amineoxide, asprosin, metformin