HPLC MS / MS analyzes of various samples carried out with nanomaterial-based microsolid phase extraction and biosensors design

he aim of this thesis is to design a device that performs microsolid phase (μ-solid phase) extraction with the help of carbon nanomaterials, and by preconcentrating some of the anti-inflammatory-analgesic drug active substances such as Ketoprofen, Naproxen, Nimesulide, Tolfenamic acid, Diclofenac in synthetic urine samples, using HPLC MS/MS device. analytical method development and validation. In addition, biosensor designs for some of these materials are also among the aims of this thesis. For this purpose, a device with two different surface areas was designed from buckypaper material and used as an extraction device. The extraction method has been optimized in terms of parameters such as different pH, mixing time and solution volume. 12 hours mixing time pH 6.2 and 50 mL digestion were determined as optimum conditions. LC- MS/MS method has been developed for post-extraction analyzes tested on artificial urine samples. According to the results obtained, the LLOQ values were for Ketoprofen, Naproxen, Nimesulide, Tolfenamic acid, Diclofenac respectively; 0.026; 0.035; 0.025; 0.031; 0.031 and 0.025ng/mL; meets the criteria in the manuals. Within the scope of our thesis work, a suitable HPLC method has been developed to investigate the DNA interaction for meclofenamic acid and flunixin substances. The method was optimized by experimenting with different columns and flow rates. The developed method has been validated and it has been concluded in the studies that there is no DNA interaction between both substances.