Investigation of physiochemical properties of plasma-activated medium

Thanks to the chemical activity of atmospheric plasmas when applied to liquids, the species formed in the liquid have broad-spectrum biological effects. The interaction of plasma discharge with liquids still has unanswered questions. These discharges, which are carried out at atmospheric pressure, can be applied to liquids superficially, or it is possible to perform the discharge in liquid. In this thesis, physicochemical changes in liquids were investigated by applying atmospheric pressure to liquids such as plasma water, remineralization solution, cell medium. When the cold atmospheric plasma formed with argon gas is applied to water, artificial fluids and cell media, the pH change and the amount of new species formed according to which parameters change were analyzed. It has been found that species such as hydrogen peroxide and nitrate are formed in the liquid. Quantities of the formed species were determined by spectrophotometric methods and test kits. It was observed that when plasma was applied to the cell medium using argon gas, the H2O2 concentrations increased according to the plasma application time, and the highest value of 7.60mg/L was reached with plasma application for 120 seconds. When H2O2 determination was made after 1 week on activated media stored at 4°C, an average of 36.58% decrease was observed in concentrations. The peroxide concentration in cell medium containing DOX was measured as 0.173mg/L in 90 seconds of plasma treatment. This value is lower than that in DOX-free cell medium (6.44 mg/liter for 90 seconds).